Humberto Boncristiani

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InstitutionResearch Associate
University of Maryland, department of entomology.
Postal address3158 Plant Sciences Bldg
College Park
United States of America
Office phone3013000499
Job /  professional statusResearcher
Preferred COLOSS theme(s)   Pests & Pathogens
COLOSS Taskforce(s)   Varroa control
Research interestsVirus-host interactions
Publications1. Chen YP, Pettis JS, Corona M, Chen WP, Li CJ, Spivak M, Visscher PK, DeGrandi-Hoffman G, Boncristiani H, Zhao Y, vanEngelsdorp D, Delaplane K, Solter L, Drummond F, Kramer M, Lipkin WI, Palacios G, Hamilton MC, Smith B, Huang SK, Zheng HQ, Li JL, Zhang X, Zhou AF, Wu LY, Zhou JZ, Lee ML, Teixeira EW, Li ZG, Evans JD. Israeli acute paralysis virus: epidemiology, pathogenesis and implications for honey bee health. PLoS Pathog. 2014 Jul
31;10(7):e1004261. doi: 10.1371/journal.ppat.1004261. eCollection 2014 Jul.
2. Li JL, Cornman RS, Evans JD, Pettis JS, Zhao Y, Murphy C, Peng WJ, Wu J, Hamilton M, Boncristiani HF Jr, Zhou L, Hammond J, Chen YP. Reply to "conclusive evidence of replication of a plant virus in honeybees is lacking". MBio. 2014 May 27;5(3):e01250-14. doi:
3. Kuster RD, Boncristiani HF, Rueppell O. Immunogene and viral transcript dynamics during parasitic Varroa destructor mite infection of developing honey bee (Apis mellifera) pupae. J Exp Biol. 2014 May 15;217(Pt 10):1710-8. doi: 10.1242/jeb.097766.
4. Li JL, Cornman RS, Evans JD, Pettis JS, Zhao Y, Murphy C, Peng WJ, Wu J, Hamilton M,
Boncristiani HF Jr, Zhou L, Hammond J, Chen YP. Systemic spread and propagation of a
plant-pathogenic virus in European honeybees, Apis mellifera. MBio. 2014 Jan 21;5(1):e00898-
13. doi: 10.1128/mBio.00898-13.
5. Boncristiani HF, Evans JD, Chen Y, Pettis J, Murphy C, Lopez DL, Simone-Finstrom M, Strand M, Tarpy DR, Rueppell O. In vitro infection of pupae with Israeli acute paralysis virus suggests disturbance of transcriptional homeostasis in honey bees (Apis mellifera). PLoS One. 2013 Sep
5;8(9):e73429. doi: 10.1371/journal.pone.0073429. eCollection 2013.
6. Wagoner KM, Boncristiani HF, Rueppell O. Multifaceted responses to two major parasites in the honey bee (Apis mellifera). BMC Ecol. 2013 Jul 17;13:26. doi: 10.1186/1472-6785-13-26.
7. Cornman RS, Boncristiani H, Dainat B, Chen Y, vanEngelsdorp D, Weaver D, Evans JD.
Population-genomic variation within RNA viruses of the Western honey bee, Apis mellifera, inferred from deep sequencing. BMC Genomics. 2013 Mar 7;14:154. doi: 10.1186/1471-2164-
8. Teixeira, E.W.1, Chen, Y.2, Message, D.3, Boncristiani, H.F.4, Pettis, J. 2, Evans, J.D. “Israeli acute paralysis virus in Africanized honey bees in southeastern Brazilian Apiaries”. Journal of Economic Entomology (2012),51(3) 282–284. doi:10.3896/IBRA.
9. Boncristiani, H, Robyn Underwood, Ryan Schwarz, Jay Evans, Jeffery Pettis, Dennis vanEngelsdorp. Direct effect of acaricides on pathogen loads and gene expression levels of honey bee Apis mellifera. Journal of Insect Physiology. (2011) 58(5):613-20.
10. Peng, W., Li, J., Boncristiani, H., Strange, J. P., Hamilton, M., & Chen, Y. (2011). Host range
expansion of honey bee Black Queen Cell Virus in the bumble bee, Bombus huntii. Apidologie,
42(5), 650–658. doi:10.1007/s13592-011-0061-5.
11. Li, J., Peng, W., Wu, J., Strange, J. P., Boncristiani, H., & Chen, Y. (2011). Cross-Species Infection of Deformed Wing Virus Poses a New Threat to Pollinator Conservation. Journal of Economic Entomology, 104(3), 732–739. doi:10.1603/EC10355.
12. Boncristiani, H., Li, J., Evans, J. D., Pettis, J., & Chen, Y. (2011). Scientific note on PCR inhibitors in the compound eyes of honey bees, Apis mellifera. Apidologie, 42(4), 457–460. doi:10.1007/s13592-011-0009-9.
13. Di Prisco, G., Pennacchio, F., Caprio, E., Boncristiani, H. F., Evans, J. D., & Chen, Y. (2011).
Varroa destructor is an effective vector of Israeli acute paralysis virus in the honeybee, Apis mellifera, Journal of General Virology, 92(Pt 1), 151–155. doi:10.1099/vir.0.023853-0.

14. Boncristiani, H.F. Rossi, R.D., Criado, M.F. Furtado, F.M. & Arruda, E. Magnetic purification of biotinylated cDNA removes false priming and ensures strand-specificity of RT-PCR for enteroviral RNAs. Journal of Virological Methods. 2009 vol. 161 (1) pp. 147-53.
15. Yanping Chen, Jay D Evans, Liang Zhou, Humberto Boncristiani, Kiyoshi Kimura, Tieguang Xiao, A M Litkowski, Jeffery S Pettis. Asymmetrical coexistence of Nosema ceranae and Nosema apis in honey bees. Journal of Invertebrate Pathology. 2009 vol. 101 (3) pp. 204-9.
16. Boncristiani HF, Di Prisco G., Pettis J., Hamilton M., Chen Y. Molecular approaches to the analysis of deformed wing virus replication and pathogenesis in the honey bee, Apis mellifera. Virol J (2009) vol. 6 pp. 221.
17. Jay D. Evans, Humberto Boncristiani, Yanping Chen. Scientific note on mass collection and hatching of honey bee embryos. Apidologie, 2009.
18. Boncristiani, H. F., & Arruda, E. (2003). Molecular cloning and expression of recombinant VP1 of coxsackievirus B5. Medicina (Ribeirao Preto), 36(1), 55–56. Ribeirão Preto. Retrieved from