Raquel Martín-Hernández

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InstitutionCentro Apícola de Marchamalo
Junta de Castilla - La Mancha
Postal addressCamino de San Martín s/n
Office phone+34949885014
Job /  professional statusResearcher
Preferred COLOSS theme(s)   Pests & Pathogens
COLOSS Project(s)   Monitoring
COLOSS Taskforce(s)   Varroa control
Research interestsBee health
PublicationsRefereed Journal articles (JCR)
1. Martín Hernández, R.; Cuéllar del Hoyo, C.; Olmeda García, A.S.; Rodríguez Rodríguez, J.A. (1994). Immunological response in rabbits infested with Rhipicephalus sanguineus. Medical and Veterinary Entomology,8:, 238-244.
2. Martín Hernández, R.; Cuéllar del Hoyo, C.; Olmeda García, A.S.; Rodríguez Rodríguez, J.A. (1995). Analysis of stage-specific and shared antigens derived from Rhipicephalus sanguineus by electrophoresis and westernblotting. Medical and Veterinary Entomology, 9:, 358-364.
3. Meana A, Pato NF, Martin R, Mateos A, Perez-Garcia J, Luzon M. Epidemiological studies on equine cestodes in central Spain: infection pattern and population dynamics. Veterinary Parasitology 2005, 130(3-4):233-40
4. Higes, M.; Martín, R.; Meana, A. (2006). Nosema ceranae, a new Microsporidian parasite in honeybees in Europe. Journal of Invertebrate Pathology, 92, 81-83.
5. Fries, I., Martín R., Meana A., García-Palencia P., Higes, M. (2006). Natural infections of Nosema ceranae in European honey bees. Journal of Apicultural Research, 45(3), 230-233.
6. Higes, M., García-Palencia P., Martín-Hernández R., Meana A. (2007). Experimental infection of Apis mellifera with Nosema ceranae (Microsporidia). Journal of Invertebrate Pathology, 94, 211–217.
7. Chauzat M.-P., Higes M., Martín-Hernández R., Meana A., Cougoule N. and Faucon J.-P. (2007). Presence of Nosema ceranae in French honeybee colonies. Journal of Apicultural Research, 46(2), 127-128.
8. Martín-Hernández R., Meana A., Prieto L., Martínez Salvador A., Garrido-Bailón E., Higes M. (2007). Outcome of colonization of Apis mellifera by Nosema ceranae. Applied and Environmental Microbiology, 73 (20), 6331-6338.
9. Martín-Hernández R., Higes M., Pérez J.L., Nozal M.J., Gómez L., Meana A. (2007). Short term negative effect of oxalic acid in Apis mellifera iberiensis. Spanish Journal of Agricultural Research, 5 (4): 474-480.
10. Higes, M., Martín-Hernández R., Garrido-Bailón E., García-Palencia P., Meana A. (2008). Detection of infective Nosema ceranae (Microsporidia) spores in corbicular pollen of forager honey bees. Journal of Invertebrate Pathology, 2007, 76–78.
11. Higes M., Martín-Hernández R., Garrido-Bailón E., Botías C., García-Palencia P., Meana A. (2008). Regurgitated pellets of Merops apiaster as fomites of infective Nosema ceranae (Microsporidia) spores. Environmental Microbiology, 10(5):1374-9.
12. Nozal MA, Bernal JL, Martín MA, Bernal J, Alvaro A, Martín R., Higes M. Trace analysis of fumagillin in honey by liquid chromatography-diode array-electrospray ionization mass spectrometry. Journal of Chromatography A. 1190 (2008), 224–231
13. Higes M., Martín-Hernández R., Botías C., Garrido-Bailón E., González-Porto A.V., Barrios L., del Nozal M.J., Bernal, J.L., Jiménez J.J.García-Palencia P., Meana A. (2008). How natural infection by Nosema ceranae causes honeybee colony collapse. Environmental Microbiology, 2008, 10(10), 2659–2669.
14. Muñoz I., Garrido E., Martín-Hernández R., Meana A., Higes M., De la Rúa P. Genetic profile of Varroa destructor infesting Apis mellifera iberiensis colonies. Journal of Apicultural Research and Bee World 2008, 47(4).
• Abstract in: Nature Reviews Microbiology, 2008, 6 (9), 650-651.
15. Olmeda A.S., Pérez J.L., Martín-Hernández R., Torrente M., Valcarcel F. Toxicity of Oxalic Acid Against Adult Hyalomma lusitanicum Ticks (Ixodida: Ixodidae) in laboratory Conditions: LD50. Journal Medical Entomology 45(4): 715-719 (2008).
16. Higes M., Martín-Hernández R., González-Porto A.V., García-Palencia P., del Nozal M.J., Mayo R., Bernal, J.L., Meana A. Honey bee colony collapse due to Nosema ceranae: A case report. Environmental Microbiology Reports 2009, 1(2), 110–113.
17. Plischuk S., Martín-Hernández R., Prieto L. Botías C., Lucía M., Meana A., Abrahamovich A.H., Lange C., Higes M. South American native bumblebees (Hymenoptera: Apidae) infected by Nosema ceranae (Microsporidia), an emerging pathogen of honey bees (Apis mellifera). Environmental Microbiology Reports 2009, 1(2), 131–135.
18. Antúnez K., Martín-Hernández R., Prieto L., Meana A., Zunino A., Higes M. Immune suppression in the honey bee (Apis mellifera) following infection by Nosema ceranae (Microsporidia). Environmental Microbiology 2009, 11(9):2284-90.
19. Martín-Hernández R., Meana A., García-Palencia P., Marín P., Botías C., Garrido-Bailón E., Barrios L., Higes M. Effect of Temperature on the biotic potential of Honeybee Microsporidia. Applied and Environmental Microbiology 2009, 75, 8, 2554–2557.
20. Higes M., Martín-Hernández R., Garrido-Bailón E., Botías C, Meana A. The presence of Nosema ceranae (Microsporidia) in North African honey bees (Apis mellifera intermissa). Journal of Apicultural Research 2009, 48, 3, 217-219.
21. Higes M., Martín-Hernández R., García-Palencia P., Marín P., Meana A. Horizontal transmission of Nosema ceranae (Microsporidia) from worker honey bees to queens (Apis mellifera). Environmental Microbiology Reports 2009, Vol 1, 495–498,
22. Higes M., Martín-Hernández R., Martínez-Salvador A, Garrido-Bailón E., González-Porto A.V., Meana A., Bernal JL, del Nozal M.J., Bernal, J. A preliminary study of the epidemiological factors related to honey bee colony loss in Spain. Environmental Microbiology Reports 2010, (2) 243-250.
23. Fenoy S., Rueda C., Higes M., Martín-Hernández R., del Águila C. High-Level resistance of Nosema ceranae, a parasite of the honeybee, to temperature and desiccation. Applied & Environmental Microbiology, 2009; .75(21), 6886–6889.
24. Meana A., Martín-Hernández R., Higes M. Reliability of spore count for the diagnosis of Nosema ceranae infection. Journal of Apicultural Research and Bee World 2010. 49(2): 212-214.
25. Higes M., Martín-Hernández R., Meana A. Nosema ceranae in Europe: the background and an update of emergent nosemosis type C. Apidologie, 2010, 41: 3, 375-392.
26. Higes M., García-Palencia P., Marín P., Botías C., Meana A., Martín-Hernández R. Differential development of microsporidia infecting honey bee (Apis mellifera) workers under increasing incubation temperature. Environmental Microbiology Reports 2010, 2: 6, 745–748.
27. García-Palencia P., Martín-Hernández R., Amelia-Virginia González-Porto Pilar Marin, Meana A., Higes M. Natural infection by Nosema ceranae causes similar lesions as in experimentally infected caged-worker honey bees (Apis mellifera). Journal of Apicultural Research 2010, 49(3): 278-283.
28. Garrido-Bailón E., Martín-Hernández R., Bernal J., Jiménez J.J., Bernal J.L., Martínez-Salvador A., Barrios L., Meana A., Higes M. The detection of Israeli Acute Paralysis virus (IAPV), fipronil and imidacloprid in professional apiaries are not related with massive honey bee colony loss in Spain. Spanish Journal of Agricultural Research 2010, Vol. 8 (3), 658-661.
29. Bernal J., Garrido-Bailón E., del Nozal MJ, González-Porto AV, Martín-Hernández R., Jiménez J.J., Bernal J.L, Higes M. The stability and effectiveness of fumagillin in controlling Nosema ceranae (Microsporidia) infection in honey bees (Apis mellifera) in laboratory and field conditions. Apidologie, (2011) 42:364–377.
30. Valera F.; Martín-Hernández R., Higes M. Evaluation of large-scale dissemination of Nosema ceranae spores by European bee-eaters Merops apiaster. Environmental Microbiology Reports 2011, 3(1), 47–53.
31. Borneck R., Viry A., Martín-Hernández R., Higes M. Honey bee colony losses in the Jura Region (France) and related pathogens. Journal of Apicultural Research and Bee World 2010, 49(4): 334-336.
32. Bernal J., Garrido-Bailón E., del Nozal MJ, González-Porto AV, Martín-Hernández R., J. C. Diego, Jiménez J.J., Bernal J.L, Higes M. Overview of Pesticide Residues in Stored Pollen and Their Potential Effect on Bee Colony (Apis mellifera) Losses in Spain. Journal of Economic Entomology 2010, 103(6): 1964-1971.
33. Sagastume S.; del Águila C., Martín-Hernández, R., Higes M., Henriques-Gil. N. Polymorphism and recombination for rDNA in the putatively asexual microsporidian Nosema ceranae, a pathogen of honeybees. Environmental Microbiology 2011, 13(1), 84–95.
34. Martín-Hernández R, Botías C, Barrios L, Martínez-Salvador A, Meana A, Mayack C, Higes M. Comparison of the energetic stress associated with experimental Nosema ceranae and Nosema apis infection of honey bees (Apis mellifera). Parasitology Research 2011, 109:605–612.
35. Bernal J., Martin-Hernandez R; J., Diego J.C., Nozal M.J., Gozalez-Porto A.V, Bernal J.L., Higes M. An Exposure study to asses the potential impact of Fipronil in treated sunflower seeds on honey bee colony losses in Spain. Pest Management Science, 2011 Oct; 67(10):1320-31.
36. Bernal J., Martín, M.T. Toribio L., Martín-Hernández R., Higes M, Bernal J.L., Nozal M.J. Determination of tylosins A, B, C and D in bee larvae by liquid chromatography coupled to ion trap-tandem mass spectrometry. Journal of Chromatography B, 879 2011, 1596–1604.
37. Botías C.;, Martín-Hernández R.; Garrido-Bailón E.; González Porto A., Martínez-Salvador A., de la Rúa P., Meana A.; Higes M. The growing prevalence of Nosema ceranae in honey bees in Spain, an emerging problem for the last decade. Research in Veterinary Sciences 2012, 93, 150–155.
38. Botías C.; Martín-Hernández R.; Barrios L.; Garrido-Bailón E.; Nanetti A.; Meana A.; Higes M. Nosema spp. parasitization decreases the effectiveness of acaricide strips (Apivar ®) in treating varroosis of honey bee (Apis mellifera iberiensis) colonies. Environmental Microbiology Reports 2012, 4 (1), 57–65.
39. Martín-Hernández R., Botías C., Garrido Bailón E., Martínez-Salvador A., Prieto L., Meana A., Higes M. Microsporidia infecting Apis mellifera: coexistence or competition. Is Nosema ceranae replacing Nosema apis? Environmental Microbiology 2012, 14(8), 2127–2138.
40. Botías C.; Martín-Hernández R.; Dias J., García-Palencia P., Matabuena M., Juarranz A., Barrios L., Meana A.; Nanetti A.; Higes M. The effect of induced queen replacement on Nosema spp. infection in honey bee (Apis mellifera iberiensis) colonies. Environmental Microbiology 2012, 14, 845-859
41. Botías C.; Martín-Hernández R.; Meana A.; Higes M. Critical aspects of the Nosema spp. diagnostic sampling in honey bee (Apis mellifera L.) colonies. Parasitology Research, DOI 10.1007/s00436-011-2760-2.
42. van der Zee R., Pisa L, Andonov S, Brodschneider R, Charrière JD, Chlebo R., Coffey MF, Crailsheim K, Dahle B, Gajda A., Gray A., Drazic MM., Higes M., Kauko L., Kence A., Kence M., Kezic N., Kiprijanovska H., Kralj J.,Kristiansen P., Martin Hernandez R., Mutinelli F., Nguyen BK., Otten C., Özkırım A., Pernal SF., Peterson M., Ramsay G., Santrac V., Soroker V., Topolska G., Uzunov A., Vejsnæs F., Wei S., Wilkins S. Managed honey bee colony losses in Canada, China, Europe, Israel and Turkey, for the winters of 2008-2009 and 2009–2010. Journal of Apicultural Research 2012, 51(1): 100-114
43. Ares A.M., Nozal M.J., Bernal J.L., Martín-Hernández R., Higes M., Bernal J. Liquid chromatography coupled to ion trap-tandem mass spectrometry to evaluate juvenile hormone III levels in bee hemolymph from Nosema spp. infected colonies. Journal of Chromatography B, 2012; http://dx.doi.org/10.1016/j.jchromb.2012.05.016
44. Jara L., Cepero A., Garrido-Bailón R., Martín-Hernández R., Higes M., De la Rúa P. Linking evolutionary lineage with the parasite prevalence in the Iberian honey bee. Journal of Invertebrate pathology 2012, 110 (1):8-13.
45. Botías C.; Anderson D.L.; Meana A.; Garrido-Bailón E.; Martín-Hernández R.; Higes M. Further Evidence of an Oriental Origin for Nosema ceranae. Journal of Apicultural Research 2012, 110, 108-113. http://dx.doi.org/10.1016/j.jip.2012.02.014
46. Dussaubat C., Brunet J.L., Higes M., Colbourne J., Lopez J., Choi J.H, Martín-Hernández R., Botías C., Cousin M., Belzunces L., Le Conte Y., Alaux C. Gut pathology and responses to the microsporidium Nosema ceranae in the honey bee Apis mellifera. PlosOne 7 (5) e37017.
47. Antúnez K., Anido M., Garrido-Bailón E., Botías C., Zunino P., Martínez-Salvador A., Martín-Hernández R., Higes M. Low prevalence of honeybee viruses in Spain during 2006 and 2007. Research in Veterinary Science, http://dx.doi.org/10.1016/j.rvsc.2012.03.006
48. Dussaubat C., Sagastume S., Gómez-Moracho T., Botías C., García-Palencia P., Martín-Hernández R., Le Conte Y., Higes M. Comparative study of Nosema ceranae isolates from two different geographic origins and of their effects on Apis mellifera iberiensis. Veterinary Microbiology, doi:10.1016/j.vetmic.2012.09.012.
49. Garrido-Bailón E., Bartolomé C., Prieto L., Botías C., Martínez-Salvador A., Meana A., Martín-Hernández R. , Higes M. The prevalence of Acarapis woodi in Spanish honey bee (Apis mellifera) colonies. Experimental Parasitology, 2012, 132:530-536.
50. Higes M., Meana A., Bartolomé C., Botías C., Martín-Hernández R. Nosema ceranae (Microsporidia), a controversial 21st century honey bee pathogen. Environmental Microbiology Reports 2013, 5 (1), 17–29.
51. Fries I., Chauzat M.P., Chen Y.P., Doublet V., Genersch E., Gisder S., Higes M., McMahon D.P., Martín-Hernández R., Natsopoulou M., Paxton R.J., Tanner G., Webster T.C., Williams G.R. Standard methods for Nosema research. Journal of Apicultural Research 2013, 52(1) DOI 10.3896/IBRA.
52. Botías C., Martín-Hernández R., Barrios L., Meana A., Higes M. Nosema spp. infection and its negative effects on honey bees (Apis mellifera iberiensis) at the colony level. Veterinary Research, accepted on 2013-2-8.
53. Martin S.J., Hardy J., Villalobos E., Martín-Hernández R., Nikaido S., Higes M. Do the honeybee pathogens Nosema ceranae and Deformed Wing Virus act synergistically? Environmental Microbiology Reports 2013, doi:10.1111/1758-2229.12052.
54. Higes M., Juarranz A., Dias-Almeida J., Matabuena M., Botías C, Meana A., García-Palencia P., Martín-Hernández R. Apoptosis in the pathogenesis of Nosema ceranae (Microsporidia: Nosematidae) in honey bees (Apis mellifera). Environmental Microbiology Reports, 2013, doi:10.1111/1758-2229.12059.
55. Garrido-Bailón E., Martínez-Salvador A., Antúnez K., Botías C., Meana A., Prieto L., Martín-Hernández R., Higes M. The prevalence of the honeybee brood pathogens Ascosphaera apis, Paenibacillus larvae and Melissococcus plutonius in Spanish apiaries determined with a new multiplex PCR assay. Microbial Biotechnology 2013, 6(6):731-9. doi: 10.1111/1751-7915.12070.
56. Higes M., Gómez-Moracho T., Botías C., Martín-Hernández R. The effect of an experimental treatment with “Nozevit®”, (a phyto-pharmacological preparation) for Nosema ceranae, infected honey bees in a Mediterranean country. Journal of Apicultural Research, accepted on 3-7-2013.
57. Sagastume S., Martin-Hernandez R., Higes M., Henriques-Gil N. Ribosomal Gene Polymorphism in Small Genomes: Analysis of Different 16S rRNA Sequences Expressed in the Honeybee Parasite Nosema ceranae (Microsporidia). Journal of Eukaryotic Microbiology, accepted on 14-8-2013.
58. Gómez-Moracho T, Maside X, Martín-Hernández R, Higes M, Bartolomé C. High levels of genetic diversity in Nosema ceranae within Apis mellifera colonies Parasitology 2013, 15, 1-7. doi:10.1017/S0031182013001790.
59. Van der Zee R; Gómez-Moracho T; Pisa L.; Sagastume, S, García-Palencia P; Maside X; Bartolomé C; Martín-Hernández R; Higes M. Virulence and polar tube protein genetic diversity of Nosema ceranae (Microsporidia) field isolates from Northern and Southern Europe in honey bees (Apis mellifera iberiensis). Environmental Microbiology Reports, Accepted on 2013-12-5; DOI: 10.1111/1758-2229.12133
60. Sánchez Collado JG, Higes M, Barrio L, Martín-Hernández R. Flow cytometry analysis of nosema species to assess spore viability and longevity. Parasitology Research, Accepted on 2014-1-29.
61. Muñoz I, Cepero A, Pinto MA, Martín-Hernández R, Higes M, De la Rúa M. Changes in Nosema incidence associated with honey bee queen introductions. Infection, Genetics and Evolution, Accepted on 2014-2-7.

Extension publications
62. Higes, M.; M.; Pérez, J.L.; Martín, R.; Sanz, A.; Rojo, N. Bernal,J.L.; Nozal, M.J.; González, MJ. Timol para el tratamiento de la varroosis. Desarrollo de un sistema de liberación controlada. Vida Apícola, 114: 44-49. 2002.
63. Meana A., Mateos A., Pato N.F., Martín R. Todo sobre los responsables de las cestodosis equinas. Equinus  3(2): 53-64. 2003.
64. Meana A., Pato N.F., Mateos A., Martín R., Luzón-Peña M. Novedades sobre las cestodosis equinas. Colección Congresos y Jornadas del IV CONGRESO INTERNACIONAL. DE MEDICINA Y CIRUGÍA EQUINA, pp.137-140. 2003.
65. Casado, M.A., Benavente A., Valcárcel F., Miró G., Martín R., Díez E., Camacho A.T., Olmeda A.S. Infestación por garrapatas en perros en España. Canis et Felis 68, 13-31. 2004.
66. Camacho A.T., Díez E., Valcárcel F., Martín R., Casado, M.A., Olmeda A.S. Babesiosis canina. Canis et Felis 68, 33-50. 2004.
67. Martín R., Díez E., Valcárcel F., Miró G., Camacho A.T., Casado M.A., Olmeda A.S. Hepatozoon canis. Canis et Felis 68, 53-62. 2004.
68. Olmeda A.S., Miró G., Camacho A.T., Díez E., Martín R., Casado, M.A., Valcárcel F. Filariosis transmitidas por garrapatas: dipetalonemosis. Canis et Felis 68, 63-74. 2004.
69. Higes Pascual M., Sanz A., Martín R., Rodrigálvarez M. Nosema apis ¿el asesino silencioso? Vida apícola: revista de apicultura, Nº. 125, 17-19. 2004
70. Higes, M., Martín, R., Sanz, A.; Alvarez, N.; Sanz, A.; Garcia, M.P.; Meana, A. El síndrome de despoblamiento de las colmenas en España. Consideraciones sobre su origen. Vida Apícola 133, 15-21. 2005.
71. Meana A., Martín Hernández R, Higes M. La silenciosa pandemia de las abejas Investigación y Ciencia, Octubre 2009.
72. Sanz, A., Higes M., Martín Hernández R. El Centro Apícola Regional de Marchamalo como referente en sanidad apícola. Agropalca, Julio-Septiembre 2009.
73. Higes Pascual M., Martín Hernández R., Sanz Herranz A. El síndrome de despoblamiento de las colmenas. Profesión Veterinaria Nº 76, Octubre- diciembre 2011.
74. Valcárcel F, Pérez Sánchez JL, Tercero Jaime JM, Basco-Basco PI, Cota Guajardo SC, Cutuli MT, Martín-Hernández R, Olmeda AS. Oxalic acid as an alternative control of host seeking ticks in field conditions. International Journal of Veterinary Medicine: Research & Reports DOI: 10.5171/2014.871622