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Standard methods for cell cultures in Apis mellifera research


Cell culture techniques are indispensable in most, if not all life science disciplines to date. Wherever appropriate cell culture models are lacking, scientific development is hampered. Unfortunately this has been and still is the case in honey bee research, because permanent honey bee cell lines have not so far been established. To overcome this hurdle, protocols for the cultivation of primary honey bee cells and of non-permanent honey bee cell lines have been developed. In addition, heterologous cell culture models for honey bee pathogens based on nonApis insect cell lines have recently been developed. To further advance this progress and to encourage bee scientists to enter the field of cell biology based research, here we present protocols for the cultivation of honey bee primary cells and non-permanent cell lines, as well as hints for the cultivation of permanent insect cell lines suitable for honey bee research.

    1. 2.1. Isolation and cultivation of primary neuronal cells
    2. 2.1.1. Protocol for pupal cells
    3. 2.1.2. Protocol for adult cells
    4. 2.2. Isolation and cultivation of primary gut cells
    5. 2.2.1. Protocol for primary gut cells
    6. 2.3. Isolation and cultivation of non-permanent cell lines
    7. 2.3.1. Protocol for preparing sterilized tissues
    8. 2.3.2. Care and observation of explanted material
    9. 2.4. Determining the viability of cultured cells
    10. 2.4.1. MTT-viability test
    11. 2.4.2. MitoTracker® Red FM-viability test for cultured cells
    1. 3.1. Available and suitable cell lines
    2. 3.2. Cultivation of insect cell lines