6.3.4. Recommendations for incubator relative humidity for maintaining adult workers in the laboratory

Considering natural colony conditions and worker preference, we recommend that adult workers of all ages should be maintained at 60-70% relative humidity in the laboratory.

Pre-trials will be needed to determine water surface area, frequency of water replacement, and choice of salt needed to sustain appropriate levels because incubator size and air exchange with the ambient surroundings will greatly influence relative humidity.