Mechanical killing

Numerous studies kill adult honey bees by physically damaging or removing an essential body section (e.g. head, thorax, or abdomen) using forceps, one’s index finger and thumb, or a scalpel. This method is relatively easy to perform, depending upon activity level and quantity of honey bees, and avoids the use of chemicals or other equipment that perhaps are not easily accessible. If there are many bees to kill, this can be a tedious method. Mechanical termination usually leaves the unaffected body part(s) intact; however, it may potentially promote parasite transmission when the exoskeleton is ruptured. The precise method of mechanical termination chosen will largely depend on the purpose of the study, but it can be tedious (Table 1).