Infection of colonies with a water spore suspension

  1.  Prepare a spore solution of 1.25 x 107 spores/ml in sterile distilled water. (See paragraph 1.4. Production and quality of inoculums)
  2.  Spray 10 ml on the each brood comb surface. This quantity has been used successfully on colonies with 8-10 combs (Flores et al., 2004a).