2.3.1. Inspection of honey bee queen shipments for small hive beetle

When receiving a shipment of honey bee queens that are to be inspected for presence of small hive beetle, the following methods can be used:

1. Remove the queen from queen cage and visually inspect her for signs of SHBs (adult, larval, or egg stages; Fig. 1-3) or any other potentially introduced species, such as the mites Tropilaelaps spp. (see the BEEBOOK paper on Tropilaelaps (Anderson et al., 2013)) or Varroa destructor (see the BEEBOOK paper on Varroa (Dietemann et al., 2013)).

2. Store queen appropriately (e.g. in a colony), according to local procedures and regulations.

3. Immediately place the shipping container and queen cage, including the worker attendants, in a sealable bag and store in a freezer for at least 24 hours to kill attendants and any introduced species such as SHB or Tropilaelaps spp.

4. Perform a second visual inspection on the queen cage, the dead worker attendants, the shipping container and other accompanying materials as quickly as possible after the freeze period.

Note: A similar protocol can be applied if inspecting shipped worker honey bees.