Detection and quantification of Melissococcus plutonius in honey bee workers exposed to European foulbrood in Czechia through conventional PCR, qPCR, and barcode sequencing
Melissococcus plutonius is the causative agent of European foulbrood (EFB), an important bacterial disease of honey bee larvae, and various methods have been developed for the detection of this disease. Adult bees are not affected but spread M. plutonius among beehives and serve as a diagnostic tool to detect EFB. Melissococcus plutonius detection based on…