Abstract Bio-analysis of insects is increasingly dependent on highly sensitive methods that require high quality biological material, such as hemolymph. However, it is difficult to collect fresh and uncontaminated hemolymph from adult bees since they are very active and have the potential to sting, and because hemolymph is rapidly melanized. Here we aimed to develop…
Abstract During the 2000s, the problem of pesticide poisoning of honeybees seemed to be almost solved. The number of cases has decreased in comparison to the 1970s. The problem of acute honeybee poisoning, however, has not disappeared, but instead has transformed into a problem of poisoning from ‘traditional’ pesticides like organophosphorus pesticides or pyrethroids, to…
Abstract Study combines data about the exposure of honeybees to pesticides from plant protection products and veterinary medicinal products. Residues of 200 pesticide and pesticide metabolites in 343 live and 74 poisoned honeybee samples, obtained during the years of 2014–2015, were determined by LC-MS/MS and GC-MS/MS. In 44% of live honeybee 48 different pesticide residues…
Abstract The effect of sublethal doses of imidacloprid on protein content and activity of proteases on honey bees was analyzed. The study was conducted in three experimental groups: colonies from groups BE-5 and BE-200 were contaminated with 5 and 200 ppb of imidacloprid, respectively, via their food supply (syrup and pollen), while group BE was…
Abstract The presence of nanoparticles on the body of the honeybee Apis dorsata Fabricius, was investigated for the frst time to better understand the bee’s behaviour. These have been observed by using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and confrmed by Atomic Force Microscopy (AFM). Our study clearly denotes that the Indian rock honey bee Apis…
Abstract In this study, different context-dependent effects of imidacloprid exposure on the honey bee response were studied. Honey bees were exposed to different concentrations of imidacloprid during a time period of 40 days. Next to these variables, a laboratory-field comparison was conducted. The influence of the chronic exposure on gene expression levels was determined using…
Abstract Wax moth larvae (Lepidoptera: Pyralidae) are serious pests on wax combs outside and inside of beehives. Management of wax moths is particularly difficult inside hive bodies housing weak colonies. In this study, five traps were designed and evaluated for their efficacy to attract mature larvae inside beehives. These traps were: (1) mesh envelope trap…
Abstract The liquid form of honey is relatively undesirable because honey could be crystallized and then spoilage under certain conditions. In this study, producing honey jelly from liquid citrus, clover and cotton honey using gelatin was investigated. Only gelatin and water were used to produce the honey jelly without other additives. Some chemical components were…
Abstract Various treatments are currently available for the control of Varroa mites. Some of these treatments depend on using natural and nonchemical compounds. In this study, the impacts of some materials (oxalic acid, thymol, and potassium citrate) on the survival of honey bees and some quality parameters were investigated under laboratory conditions. The potential impacts…
Abstract We measured the distribution of sugar solution within groups of caged honey bees (Apis mellifera) under standard in vitro laboratory conditions using 14C polyethylene glycol as a radioactive marker to analyze ingestion by individual bees after group feeding. We studied the impact of different experimental setups by varying the number of bees, age of bees, origin of…