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The Fate of Major Royal Jelly Proteins during Proteolytic Digestion

Royal jelly (RJ) is a beehive product with a complex composition, major royal jelly proteins (MRJPs) being the most abundant proteins. Cell culture and animal studies suggest various biological activities for the full-length/native MRJPs. In the field of apitherapy, it is assumed that MRJPs can positively affect human health. However, whenever RJ is administered orally,…

How Honeybees Defy Gravity with Royal Jelly to Raise Queens

The female sex in honeybees (Apis spp.) comprises a reproductive queen and a sterile worker caste. Nurse bees feed all larvae progressively with a caste-specific food jelly until the prepupal stage. Only those larvae that are exclusively fed a large amount of royal jelly (RJ) develop into queens. RJ is a composite secretion of two…

The effect of Agaricus brasiliensis extract supplementation on honey bee

ABSTRACT This study was done to discover any beneficial effect of a medicinal mushroom Agaricus brasiliensis extract on the honey bee. Firstly, a laboratory experiment was conducted on 640 bees reared in 32 singleuse plastic rearing cups. A. brasiliensis extract proved safe in all doses tested (50, 100 and 150 mg/kg/ day) irrespective of feeding…

Perfect Match: Simultaneous Strawberry Pollination & Bio-Sampling of the Plant

Abstract In this study we show that honey bee colonies placed in a greenhouse for pollination of strawberry can simultaneously be used to indicate the presence of the plant pathogenic bacterium Erwinia pyrifoliae. This was demonstrated by using two methods of qualitative sacrificial and non-sacrificial bio sampling of the honey bee colony. A novel method…

Monitoring of Small Hive Beetle (Aethina Tumida Murray) in Calabria

Abstract The Small Hive Beetle (SHB), Aethina tumida, is an invasive pest of honey bee colonies that causes significant damage to the beekeeping sector. SHB was detected in southern Italy (EU) in 2014 and despite adopted eradication measures, is still present there. After three years of observations of SHB in Calabria (2014-2016), we provide here…

Liquid formic acid 60% to control varroa mites

Abstract We verified the acaricide efficacy on Varroa destructor and the toxicity on honey bees of summer treatments carried out in three different apiaries in Central Italy with an evaporator filled with 290 ml of formic acid 60%. Acaricide efficacy was evaluated counting the number of mites killed by the application of formic acid in the…

Detection of the desiccant and plant growth regulator

We here describe the results of the investigation of a honeybee hive that exhibited symptoms of poisoning in the July 2013. During the summer, only a few pesticides such as desiccants, typically quaternary ammonium (quat) and glyphosate formulations, are applied. We therefore analysed samples of not only a wide range of pesticides, but also desiccants. Additionally,…

Presence of Apis Rhabdovirus-1 in Populations of Pollinators

We describe the identification by a metagenomics approach of Apis rhabdovirus, ARV-1, in A. mellifera, Bombus impatiens and in V. destructor mites from populations collected in the US and in Israel.  By  RT-PCR we found that  ARV-1 can reach high titers of  107–108 viral genomic copies per individual in individual honey bees and mites . ARV-1…

Two new articles published

Two new articles of our team: New article 1. Vejnovic Branislav, Stevanovic Jevrosima, Schwarz Ryan S, Aleksic Nevenka, Mirilovic Milorad, Jovanovic Nemanja M, Stanimirovic Zoran (2017) Quantitative PCR assessment of Lotmaria passim in Apis mellifera colonies co-infected naturally with Nosema ceranae. Journal of Invertebrate Pathology, doi: 10.1016/j.jip.2017.11.003. https://www.sciencedirect.com/science/article/pii/S0022201117303245 New article 2. Glavinic Uros, Stankovic Biljana,…

Neonicotinoid insecticides in pollen, honey and adult bees

Honeybee losses have been attributed to multiple stressors and factors including the neonicotinoid insecticides (NIs). Much of the study of hive contamination has been focused upon temperate regions such as Europe, Canada and the United States. This study looks for the first time at honey, pollen and bees collected from across the Nile Delta in…