Pingli Dai

<strong>RESEARCH PROGRAM</strong>

Research within 3 general categories:

<strong>1) Effects of Pesticides on Honey Bees.</strong> Study on the sublethal effects of bifenthrin and deltamethrin on fecundity, growth, and development of <em>Apis mellifera</em>. We research the effects of bifenthrin, deltamethrin and fluvalinate on excitability of the isolated neurons by the patch-clamp.

<strong>2) Effects of Transgenic crop on Honey Bees.</strong> We research the effects of the Cry1Ah toxin and Bt corn pollen on honeybees, including Bt cry1Ah toxin on workers of <em>Apis mellifera</em> and <em>Apis cerana</em>, Bt cry1Ah corn pollen on larvae of <em>Apis mellifera</em> and <em>Apis cerana, </em>and effects of transgenic Bt cry1Ah corn on Apis mellifera colony in field cages.

<strong>3) Honeybees pests.</strong> Study on identification, distribution and management of Varroa, Tropilaelaps, Nosema. Study on diagnosis of virus. We breed great wax moth in the laboratory and evaluated Bt toxin as a potential control for wax moth larvae in laboratory.

<strong>RESEARCH PUBLICATIONS</strong>

[1] Yang Y, Ma SL, Yan ZX, Liu F, Diao QY, <strong>Dai PL</strong>*. Effects of three common pesticides on survival, food consumption and midgut bacterial communities of adult workers <em>Apis cerana</em> and <em>Apis mellifera</em>. Environmental Pollution, 2019, 249: 860-867.

[2] <strong>Dai PL</strong>*, Wang MY, Geng LL, Yan ZX, Yang Y, Guo R, Ma SL, Diao QY*. The effect of Bt Cry9Ee toxin on honey bee brood and adults reared in vitro, <em>Apis mellifera</em> (Hymenoptera: Apidae). Ecotoxicology and Environmental Safety, 2019, 181: 381-387.

[3] <strong>Dai PL*</strong>, Yan ZX, Ma SL, Yang Y, Wang Q, Hou CS, Wu YY, Liu YJ, Diao QY<strong>*</strong>. The herbicide glyphosate negatively affects midguts bacterial communities and survival of honey bee during larvae reared in vitro. Journal Agricultural and Food Chemistry, 2018, 66: 7786-7793.

[4] <strong>Dai PL*</strong>, Jack CJ, Mortensen AN, Bustamante TA, Bloomquist JR, Ellis JD<strong>*</strong>. Chronic toxicity of clothianidin, imidacloprid, chlorpyrifos, and dimethoate to <em>Apis mellifera</em> L. larvae reared in vitro. Pest Management Science, 2018, DOI: 10.1002/ps.5124

[5] <strong>Dai PL*</strong>, Jack CJ, Mortensen AN, Bloomquist JR, Ellis JD<strong>*</strong>. The impacts of chlorothalonil and diflubenzuron on <em>Apis mellifera</em> L. larvae reared in vitro. Ecotoxicology and Environmental Safety, 2018, 164: 283-288.

[6] <strong>Dai PL*</strong>, Jack CJ, Mortensen AN, Bustamante TA, Ellis JD<strong>*</strong>. Chronic toxicity of amitraz, coumaphos and fluvalinate to <em>Apis mellifera</em> L. larvae reared in vitro. Scientific Reports, 2018, 8:5635.

[7] Jia HR, <strong>Dai PL*</strong>, Geng LL, Jack CJ, Li YH, Wu YY, Diao QY, Ellis JD. No effect of Bt Cry1Ie toxin on bacterial diversity in the midgut of the Chinese honey bees, <em>Apis cerana cerana</em> (Hymenoptera, Apidae). Scientific Reports, 2017, 7:41688.

[8] <strong>Dai PL*</strong>, Jack CJ, Mortensen AN, Ellis JD*. Acute toxicity of five pesticides to <em>Apis mellifera</em> larvae reared in vitro. Pest Manag Sci, 2017, 73：2282-2286.

[9] <strong>Dai PL*</strong>, Jia HR, Jack CJ, Geng LL, Liu F, Hou CS, Diao QY, Ellis J D. Bt Cry1Ie toxin does not impact the survival and pollen consumption of Chinese honey bees, <em>Apis cerana cerana </em>(Hymenoptera, Apidae). Journal of Economic Entomology, 2016, 109 (6): 2259-2263.

[10] Jia HR, Geng LL, Wang Q, Diao QY, Zhou T, <strong>Dai PL*</strong>. The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of <em>Apis mellifera ligustica</em> (Hymenoptera: Apidae). Scientific Reports, 2016, 6, 24664.

[11] <strong>Dai PL</strong>, Jia HR, Geng LL, Diao QY*. Bt toxin Cry1Ie causes no negative effects on survival, pollen consumption, or olfactory learning in worker honey Bees (Hymenoptera: Apidae).  Journal of Economic Entomology, 2016, 109 (3): 1028-1033.

[12] <strong>Dai PL</strong>, Zhou W, Zhang J, Lang ZH, Zhou T*, Wang Q, Cui HJ, Jiang WY, Wu YY. Effects of Bt cry1Ah corn pollen on immature workers of <em>Apis cerana cerana</em>. Journal of Apicultural Research, 2015, 54(1):72-76.

[13] <strong>Dai PL</strong>, Zhou W, Zhang J, Wang Q, Jiang WY, Cui HJ, Sun JH, Wu YY, Zhou T*. The effects of Bt Cry1Ah toxin on worker honeybees (<em>Apis mellifera ligustica</em> and <em>Apis cerana cerana</em>), Apidologie, 2012, 43(4), 384-391.

[14] <strong>Dai PL</strong>, Zhou W, Zhang J, Cui HJ, Wang Q, Jiang WY, Sun JH, Wu YY, Zhou T*. Field assessment of Bt <em>cry1Ah</em> corn pollen on the survival, development and behavior of <em>Apis mellifera ligustica,</em> Ecotoxicology and Environmental Safety, 2012, 79, 232-237.

[15] <strong>Dai PL</strong>, Wang Q, Sun JH, Liu F, Wang X, Wu YY, Zhou T*. Effects of sublethal concentrations of bifenthrin and deltamethrin on fecundity, growth, and development of the honeybee <em>Apis mellifera ligustica</em>. Environmental Toxicology and Chemistry, 2010, 29(3): 644-649.

<strong>MISCELLANEOUS SERVICE/WORK</strong>

I am the secretary of bee protection committee of China apicultural association, I helped director organize academic seminar each year.

I took lectures about honeybee pests for the beekeepers in China, about 2300 from Beijing, Hebei province, Shanxi province and Hubei province in recent 5 years.

As the member of bee protection lab, I help beekeepers in the diagnosis of more than 200 samples each year. I reply beekeepers questions about bee disease more than 300 times each year.